Difference between revisions of "Part:BBa K118025:Experience"
(→User Reviews) |
Simon Heinze (Talk | contribs) (→User Reviews) |
||
Line 9: | Line 9: | ||
===User Reviews=== | ===User Reviews=== | ||
<!-- DON'T DELETE --><partinfo>BBa_K118025 StartReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K118025 StartReviews</partinfo> | ||
− | + | ||
{|width='80%' style='border:1px solid gray' | {|width='80%' style='border:1px solid gray' | ||
|- | |- | ||
|width='10%'| | |width='10%'| | ||
<partinfo>BBa_K118025 AddReview number</partinfo> | <partinfo>BBa_K118025 AddReview number</partinfo> | ||
− | <I> | + | <I>iGEM TU Munich 2012</I> |
|width='60%' valign='top'| | |width='60%' valign='top'| | ||
− | + | Comment to 2011 British Columbia's review: We succesfully used the limonene-synthase which is included in this biobrick for expression in yeast. We could also detect the production of limonene in vitro and in vivo. Check out our Part <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K801060">BBa_K801060</a></html>!!! | |
|}; | |}; | ||
<!-- End of the user review template --> | <!-- End of the user review template --> |
Latest revision as of 17:38, 26 September 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K118025
Limonene synthase expression with pRARE2 in C41 DE3 E.coli that can be used to synthesize limonene terpenes.
User Reviews
UNIQ005ec6e930502058-partinfo-00000000-QINU
No review score entered. iGEM TU Munich 2012 |
Comment to 2011 British Columbia's review: We succesfully used the limonene-synthase which is included in this biobrick for expression in yeast. We could also detect the production of limonene in vitro and in vivo. Check out our Part BBa_K801060!!! |
•••••
British Columbia iGEM 2011 |
This composite part was expressed in our C41 E. coli that contained a pRARE plasmid encoding rare tRNA synthases. However, when we tried to express the limonene synthase in yeast, we were not able to obtain evidence for protein expression or function. So, we recommend that this part only be introduced to organisms capable of expressing foreign DNA. |
UNIQ005ec6e930502058-partinfo-00000004-QINU
Characterization by British Columbia iGEM 2011
GC-MS analysis of Limonene Synthase function by British Columbia iGEM 2011
The limonene generator with lac promoter (BBa_K118025) includes the limonene generator (BBa_K118024) under the control of a lac promoter (BBa_J33207). This composite part's purpose is to express the limonene synthase (BBa_I742110), which is also available with a ribosome site (BBa_I742111).