Difference between revisions of "Part:BBa K608351"
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<partinfo>BBa_K608351 short</partinfo> | <partinfo>BBa_K608351 short</partinfo> | ||
− | + | This is based on part ([https://parts.igem.org/Part:BBa_K098995 BBa_K098995]) | |
+ | |||
+ | <br> | ||
+ | |||
+ | '''Why did we make this part?''': Because sequencing of ([https://parts.igem.org/Part:BBa_K098995 BBa_K098995]) revealed the lack of nucleotides between the RBS and start codon of the temperature sensitive λ cI repressor ([https://parts.igem.org/Part:BBa_K098997 BBa_K098997]) CDS. | ||
+ | |||
− | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | This composite promoter consists of a constitutive promoter ([https://parts.igem.org/Part:BBa_J23114 BBa_J23114]), the temperature sensitive λ cI repressor protein ([https://parts.igem.org/Part:BBa_K098997 BBa_K098997]) ''which has a cI857 mutation that results in denaturation of the repressor when the temperature is raised to 42°C'', and a cI regulated promoter based on the λ pR promoter ([https://parts.igem.org/Part:BBa_R0051 BBa_R0051]). | ||
+ | <br> | ||
+ | |||
+ | {| style="color:black; width="75%" cellpadding="10%" cellpadding="15%" cellspacing="0" border="1" align=center | ||
+ | |- | ||
+ | | style="width: 50%;background-color:lightgrey;" | | ||
+ | ; [[Image:Freiburg11_Presentation1.jpg|350px]] | ||
+ | The temperature sensitive promoter functioning at 28-30°C | ||
+ | | style="width: 50%;background-color:lightgrey;" | | ||
+ | ; | ||
+ | [[Image:Freiburg11_Presentation3.jpg|350px]] | ||
+ | The temperature sensitive promoter functioning at 42°C | ||
+ | |} | ||
+ | <br> | ||
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
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− | + | ||
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+ | ==Functional Parameters: Austin_UTexas== | ||
+ | <html> | ||
+ | <body> | ||
<partinfo>BBa_K608351 parameters</partinfo> | <partinfo>BBa_K608351 parameters</partinfo> | ||
− | < | + | <h3><center>Burden Imposed by this Part:</center></h3> |
+ | <figure> | ||
+ | <div class = "center"> | ||
+ | <center><img src = "https://static.igem.org/mediawiki/parts/f/fa/T--Austin_Utexas--no_burden_icon.png" style = "width:160px;height:120px"></center> | ||
+ | </div> | ||
+ | <figcaption><center><b>Burden Value: 2.9 ± 3.8% </b></center></figcaption> | ||
+ | </figure> | ||
+ | <p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the | ||
+ | <a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the <a href="http://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team</a>.</p> | ||
+ | <p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p> | ||
+ | </body> | ||
+ | </html> |
Latest revision as of 19:03, 3 September 2020
(correct) temperature sensitive promoter
This is based on part (BBa_K098995)
Why did we make this part?: Because sequencing of (BBa_K098995) revealed the lack of nucleotides between the RBS and start codon of the temperature sensitive λ cI repressor (BBa_K098997) CDS.
Usage and Biology
This composite promoter consists of a constitutive promoter (BBa_J23114), the temperature sensitive λ cI repressor protein (BBa_K098997) which has a cI857 mutation that results in denaturation of the repressor when the temperature is raised to 42°C, and a cI regulated promoter based on the λ pR promoter (BBa_R0051).
The temperature sensitive promoter functioning at 28-30°C |
|
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NotI site found at 781 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters: Austin_UTexas
Burden Imposed by this Part:
Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.
This functional parameter was added by the 2020 Austin_UTexas team.