Difference between revisions of "Part:BBa K323135"

Revision as of 21:15, 23 October 2010

VioA and VioB enzymes fused with zinc fingers under pBAD promoter

The part contains two of the enzymes of the violacein biosynthetic pathway, fused to zinc finger proteins. VioA enzyme is linked to the Blues zinc finger (Part:BBa_K323085) and VioB enzyme to the Zif268 zinc finger (Part:BBa_K323134). When translated to protein, two functional chimeric proteins which bind to a specific DNA sequence are produced. We designed a DNA program (Part:BBa_K323066) with these sequences to bind the chimeric enzymes on a fixed distance.

In combination with Part:BBa_K323132, we have bound all of the enzymes, necessary for synthesis of violacein, on the DNA program. This enabled a faster and increased synthesis of the desired product, as the enzymes were fixed close together and the intermediates were immediately available to the next enzyme.

We have designed another DNA program (Part:BBa_K323046) with no binding site for the HivC_VioD chimeric protein. By this we showed that the program determines the final product - in our case, larger amounts of deoxyviolacein were formed.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1204
Illegal NheI site found at 2568
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1144
Illegal BamHI site found at 6132
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 5657
Illegal AgeI site found at 979
Illegal AgeI site found at 5853
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 961

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K323135 short</partinfo>
-The part contains two of the enzymes of the violacein biosynthetic pathway, fused to zinc finger proteins. VioA enzyme is linked to the Blues zinc finger ([[Part:BBa_K323085]]) and VioB enzyme to the Zif268 zinc finger (Part: BBa_K323134). When translated to protein, two functional chimeric proteins which bind to a specific DNA sequence are produced. We designed a DNA program (Part:BBa_K323066) with these sequences to bind the chimeric enzymes on a fixed distance.
+The part contains two of the enzymes of the violacein biosynthetic pathway, fused to zinc finger proteins. VioA enzyme is linked to the Blues zinc finger ([[Part:BBa_K323085]]) and VioB enzyme to the Zif268 zinc finger ([[Part:BBa_K323134]]). When translated to protein, two functional chimeric proteins which bind to a specific DNA sequence are produced. We designed a DNA program ([[Part:BBa_K323066]]) with these sequences to bind the chimeric enzymes on a fixed distance.
-In combination with part BBa_K323132, we have bound all of the enzymes, necessary for synthesis of violacein, on the DNA program. This enabled a faster and increased synthesis of the desired product, as the enzymes were fixed close together and the intermediates were immediately available to the next enzyme.
+In combination with [[Part:BBa_K323132]], we have bound all of the enzymes, necessary for synthesis of violacein, on the DNA program. This enabled a faster and increased synthesis of the desired product, as the enzymes were fixed close together and the intermediates were immediately available to the next enzyme.
-We have designed another DNA program (Part: BBa_K323046) with no binding site for the HivC_VioD chimeric protein. By this we showed that the program determines the final product - in our case, larger amounts of deoxyviolacein were formed.
+We have designed another DNA program ([[Part:BBa_K323046]]) with no binding site for the HivC_VioD chimeric protein. By this we showed that the program determines the final product - in our case, larger amounts of deoxyviolacein were formed.
 <!-- Add more about the biology of this part here