Difference between revisions of "Part:BBa K5117044"
| Line 10: | Line 10: | ||
<li>ribosome binding site of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117000">(BBa_K5117000)</a></html></li> | <li>ribosome binding site of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117000">(BBa_K5117000)</a></html></li> | ||
| − | <li>gene of the uncultured bacterium HR29 encoding a polyethylene terephthalate hydrolase (PETase, EC 3.1.1.101), a homologue of the leaf-branch compost cutinase (LCC), codon optimized for <i>Bacillus subtilis</i> (Xi <i>et al.</i> 2021) without any signal peptide,</ | + | <li>gene of the uncultured bacterium HR29 encoding a polyethylene terephthalate hydrolase (PETase, EC 3.1.1.101), </li> |
| + | |||
| + | <div style="text-indent: 1px;">a homologue of the leaf-branch compost cutinase (LCC), codon optimized for <i>Bacillus subtilis</i> (Xi <i>et al.</i> 2021) without any signal peptide,</div> | ||
<div style="text-indent: 1px;">addition of a short flexible linker (L1) downstream of the coding sequence encoding the amino acids GA <html><a href="https://parts.igem.org/Part:BBa_K5117032">(BBa_K5117032)</a></html></div> | <div style="text-indent: 1px;">addition of a short flexible linker (L1) downstream of the coding sequence encoding the amino acids GA <html><a href="https://parts.igem.org/Part:BBa_K5117032">(BBa_K5117032)</a></html></div> | ||
Revision as of 16:29, 1 October 2024
PcotYZ-BsRBS-BhrPET-L1-CotY-B0014
This part serves as transcriptional unit composed of:
- promoter PcotYZ of Bacillus subtilis (BBa_K5117021)
- ribosome binding site of Bacillus subtilis (BBa_K5117000)
- gene of the uncultured bacterium HR29 encoding a polyethylene terephthalate hydrolase (PETase, EC 3.1.1.101),
- cotY gene of Bacillus subtilis (BBa_K5117022)
- bidirectional terminator B0014 (BBa_B0014)
a homologue of the leaf-branch compost cutinase (LCC), codon optimized for Bacillus subtilis (Xi et al. 2021) without any signal peptide,
addition of a short flexible linker (L1) downstream of the coding sequence encoding the amino acids GA (BBa_K5117032)
Biosafety level: S1
Target organism: Bacillus subtilis
Main purpose of use: Immobilization of BhrPET on the spore crust of B. subtilis (spore surface display)
Application: Degradation of PET
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1009
Illegal AgeI site found at 695
Illegal AgeI site found at 740 - 1000COMPATIBLE WITH RFC[1000]