Difference between revisions of "Part:pSB1AK3"
(→Usage and Biology) |
|||
| (3 intermediate revisions by 2 users not shown) | |||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>pSB1AK3 short</partinfo> | <partinfo>pSB1AK3 short</partinfo> | ||
pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). | pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell). | ||
| + | Annotated [http://www.biology.utah.edu/jorgensen/wayned/ape/ APE] file for [https://static.igem.org/mediawiki/parts/0/03/PsB1AK3.ogg pSB1AK3] [[Image:PsB1AK3 map.jpg|thumb|center|Plasmid map for pSB1AK3]] | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | |||
Kanamycin resistant up to 5000 ug/ml. | Kanamycin resistant up to 5000 ug/ml. | ||
Tetracycline sensitive at 1.5 ug/ml Chloramphicol sensitive at 3.5 ug/ml (spayne and tk 6/6/05) | Tetracycline sensitive at 1.5 ug/ml Chloramphicol sensitive at 3.5 ug/ml (spayne and tk 6/6/05) | ||
| + | |||
| + | *Primers for these Biobrick vectors can be found in [[Part:BBa_G00100]] (aka VF2) and [[Part:BBa_G00101]] (aka. VR). See also [[Part:BBa_J18907]] (aka VR2) for lower temperature sequencing reactions. | ||
| + | |||
| + | *This vector is also available in the Fusion (Freiburg) Biobrick format: [[Part:BBa_J18902]] | ||
| Line 14: | Line 19: | ||
<partinfo>pSB1AK3 SequenceAndFeatures</partinfo> | <partinfo>pSB1AK3 SequenceAndFeatures</partinfo> | ||
| + | |||
| + | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>pSB1AK3 parameters</partinfo> | <partinfo>pSB1AK3 parameters</partinfo> | ||
| + | <!-- --> | ||
Latest revision as of 15:53, 26 February 2009
High copy BioBrick assembly plasmid
pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).
Annotated [http://www.biology.utah.edu/jorgensen/wayned/ape/ APE] file for pSB1AK3
Usage and Biology
Kanamycin resistant up to 5000 ug/ml.
Tetracycline sensitive at 1.5 ug/ml Chloramphicol sensitive at 3.5 ug/ml (spayne and tk 6/6/05)
- Primers for these Biobrick vectors can be found in Part:BBa_G00100 (aka VF2) and Part:BBa_G00101 (aka. VR). See also Part:BBa_J18907 (aka VR2) for lower temperature sequencing reactions.
- This vector is also available in the Fusion (Freiburg) Biobrick format: Part:BBa_J18902
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3168
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 3174 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3168
Illegal XhoI site found at 1035
Illegal XhoI site found at 2061 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 3168
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 3168
Plasmid lacks a suffix.
Illegal XbaI site found at 3183
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2207