Difference between revisions of "Part:BBa K157040:Design"

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===Design Notes===
 
===Design Notes===
  
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CMV Promotor is a viral regulatory element to mediate strong expression of the downstream gene sequence. CMV stands for cytomegalovirus. Because of its strong activity in most cells its a very popular choice to mediate expression of a foreign protein.
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It has a eukaryotic terminator.
  
 
===Source===
 
===Source===
  
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The Transfectionvector is according to the BioBrick 2007 part [https://parts.igem.org/Part:BBa_J52017 BBa_J52017].<br>
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The vector is derived from BioBrick part [https://parts.igem.org/Part:BBa_J52017 BBa_J52017].<br>
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CMV promotor was obtained by PCR by using the Biobrick [https://parts.igem.org/Part:BBa_J52038 BBa_J52038] as template.<br>
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===References===
 
===References===

Latest revision as of 19:28, 23 November 2008

Eukaryotic terminator vector (very similar to pSB1AK8)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3405
    Illegal XbaI site found at 3420
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3405
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 8
    Illegal NotI site found at 3411
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3405
    Illegal XhoI site found at 1272
    Illegal XhoI site found at 2298
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a suffix.
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3405
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3420
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2444


Design Notes

It has a eukaryotic terminator.

Source

The vector is derived from BioBrick part BBa_J52017.

References