Difference between revisions of "Part:BBa K157040:Design"

Revision as of 19:27, 23 November 2008

Eukaryotic terminator vector (very similar to pSB1AK8)

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3405
Illegal XbaI site found at 3420
Illegal SpeI site found at 2
Illegal PstI site found at 16
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3405
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 8
Illegal NotI site found at 3411
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3405
Illegal XhoI site found at 1272
Illegal XhoI site found at 2298
23
INCOMPATIBLE WITH RFC[23]
Plasmid lacks a suffix.
Illegal SpeI site found at 2
Illegal PstI site found at 16
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 3405
Plasmid lacks a suffix.
Illegal XbaI site found at 3420
Illegal SpeI site found at 2
Illegal PstI site found at 16
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2444

Design Notes

It has a eukaryotic terminator.

Source

The Transfectionvector is according to the BioBrick 2007 part BBa_J52017.
CMV promotor was obtained by PCR by using the Biobrick BBa_J52038 as template.

References

- W. Bruening, B. Giasson, W. Mushynski1 and H. D. Durham (1998): Activation of stress-activated MAP protein kinases up-regulates expression of transgenes driven by the cytomegalovirus immediate/early promoter, in Nucleic Acids Research, 1998, Vol. 26, No. 2

@@ Line 7: / Line 7: @@
 ===Design Notes===
-CMV Promotor is a viral regulatory element to mediate strong expression of the downstream gene sequence. CMV stands for cytomegalovirus. Because of its strong activity in most cells its a very popular choice to mediate expression of a foreign protein.
+It has a eukaryotic terminator.
 ===Source===