Difference between revisions of "Part:BBa R0065"

Latest revision as of 19:09, 27 August 2020

Promoter (lambda cI and luxR regulated -- hybrid)

cI repressor negatively regulates this promoter and LuxR activates its transcription.The effect of cI is dominant over LuxR. This part is based on the LuxR and cI repressor regulated hybrid promoter designed and tested by Ron Weiss. It requires the binding of two cI repressor dimers for maximal repression and contains two cI repressor binding sites namely, OR1 and OR2. This promoter is leaky in the sense that 'some' transcription is seen in the absence of both cI and LuxR.

LuxI	cI	activity of promoter
+	+	zero
+	-	maximum
-	+	zero
-	-	leaky (no quantitative information)

Usage and Biology

Pretty good 'off' in the absence of LuxR/HSL. [jb, 5/24/04]

very leaky (no LuxR/HSL) at high (100-300) copy number. can only be slightly induced by LuxR+3OC6HSL. (jt, ut-austin; 08/11/05)
Part has since been improved by the [http://2010.igem.org/Team:MIT 2010 MIT iGEM] team in the form of the part Part:BBa_K415032 where the second -10 promoter region has been removed, and the part is thus less leaky.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Functional Parameters: Austin_UTexas

BBa_R0065 parameters

Burden Imposed by this Part:

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_R0065 short</partinfo>
@@ Line 5: / Line 4: @@
 cI repressor negatively regulates this promoter and LuxR activates its transcription.The      effect of cI is dominant over LuxR. This part is based on the LuxR and cI      repressor regulated hybrid promoter designed and tested by Ron Weiss. It requires      the binding of two cI repressor dimers for maximal repression and contains      two cI repressor binding sites namely, OR1 and OR2. This promoter is leaky      in the sense that 'some' transcription is seen in the absence of both cI and      LuxR.
-&nbsp;   <table width="75%" border="1">     <tr>       <td><strong>LuxI</strong></td>       <td><strong>cI</strong></td>       <td><strong>activity of promoter</strong></td>     </tr>     <tr>       <td>+</td>       <td>+</td>       <td>zero</td>     </tr>     <tr>       <td>+</td>       <td>-</td>       <td>maximum</td>     </tr>     <tr>       <td>-</td>       <td>+</td>       <td>zero</td>     </tr>     <tr>       <td>-</td>       <td>-</td>       <td>leaky (no quantitative information)</td>     </tr>   </table>
+   <table width="75%" border="1">     <tr>       <td><strong>LuxI</strong></td>       <td><strong>cI</strong></td>       <td><strong>activity of promoter</strong></td>     </tr>     <tr>       <td>+</td>       <td>+</td>       <td>zero</td>     </tr>     <tr>       <td>+</td>       <td>-</td>       <td>maximum</td>     </tr>     <tr>       <td>-</td>       <td>+</td>       <td>zero</td>     </tr>     <tr>       <td>-</td>       <td>-</td>       <td>leaky (no quantitative information)</td>     </tr>   </table>
-&nbsp;
 ===Usage and Biology===
 Pretty good 'off' in the absence of LuxR/HSL. [jb, 5/24/04]
 *very leaky (no LuxR/HSL) at high (100-300) copy number.  can only be slightly induced by LuxR+3OC6HSL.  (jt, ut-austin; 08/11/05)
+* Part has since been improved by the [http://2010.igem.org/Team:MIT 2010 MIT iGEM] team in the form of the part [[Part:BBa_K415032]] where the second -10 promoter region has been removed, and the part is thus less leaky.
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_R0065 SequenceAndFeatures</partinfo>
-===Functional Parameters===
+==Functional Parameters: Austin_UTexas==
+<html>
+<body>
 <partinfo>BBa_R0065 parameters</partinfo>
+<h3><center>Burden Imposed by this Part:</center></h3>
+<figure>
+<div class = "center">
+<center><img src = "https://static.igem.org/mediawiki/parts/f/fa/T--Austin_Utexas--no_burden_icon.png" style = "width:160px;height:120px"></center>
+</div>
+<figcaption><center><b>Burden Value: 1.2 ± 2.8% </b></center></figcaption>
+</figure>
+<p> Burden is the percent reduction in the growth rate of <i>E. coli</i> cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the
+<a href="https://parts.igem.org/Part:BBa_K3174002">BBa_K3174002</a> - <a href="https://parts.igem.org/Part:BBa_K3174007">BBa_K3174007</a> pages for more information on the methods, an explanation of the sources of burden,  and other conclusions from a large-scale measurement project conducted by the <a href="http://2019.igem.org/Team:Austin_UTexas">2019 Austin_UTexas team</a>.</p>
+<p>This functional parameter was added by the <a href="https://2020.igem.org/Team:Austin_UTexas/Contribution">2020 Austin_UTexas team.</a></p>
+</body>
+</html>