Part:BBa_K415032
Improved PLux/cI-OR Promoter
This part is a promoter that was designed as a correction to the anomalous Part:BBa_R0065 BioBrick. This part consists of a Lux Box binding site for the LuxR-C6HSL Complex; in addition, it contains two operator sites for the cI protein appropriated from the Lambda bacteriophage. Because basal expression without the LuxR-C6HSL complex present is expected to be minimal, and because the cI operator sites are positioned so as to block the critical -10 transcription initiation site on the promoter, if cI is high, the promoter is off; if AHL is low and cI is absent, the promoter is leaky; if AHL is high and cI is absent, the promoter is on.
Slightly weaker binding than Part:BBa_K415031.
K415032 is less leaky than R0065 |
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| The above images are FACS scatter plots of co-transformations of either pTSMa (Part:BBa_K415300) and Part:BBa_K415069 or pTSMa and Part:BBa_K415023. The x-axis represents the amount of green fluorescence, and the y-axis represents the amount of red fluorescence. The parts K415069 and K415023 differ only in a single promoter sequence: K415069 has the K415031 promoter in place of a single R0065 promoter in the K415023 sequence. In this way, the more "leaky" the promoter, the more red fluorescing cells.
As you can see in the images, fewer cells with pTSMa+K415069 are leaky: 3% of total cells with pTSMa+K415069 fluoresce red, and 15% of total cells with pTSMa+K415023 fluoresce red. |
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |