Difference between revisions of "Part:BBa K3110040:Design"

(→‎Design Notes)
(→‎References)
 
(One intermediate revision by the same user not shown)
Line 9: Line 9:
 
This part contains:
 
This part contains:
 
<ul>
 
<ul>
−
<li>The Promoter and Operator Region: lldRO1-J23117-lldRO2 ([[Part:BBa_K1847008]])
+
<li>The Promoter and Operator Region: lldRO1-J23117-lldRO2 ([[Part:BBa_K1847008]]) -designed by iGEM team of ETH Zurich -2015
 
<li>A strong RBS: ([[Part:BBa_B0034]])
 
<li>A strong RBS: ([[Part:BBa_B0034]])
 
<li>Reporter Protein: sfGFP ([[Part:BBa_I746916]])
 
<li>Reporter Protein: sfGFP ([[Part:BBa_I746916]])
Line 20: Line 20:
  
 
===References===
 
===References===
 +
<ul>
 +
<li>parts.igem.org/Part:BBa_K1847008
 +
<li>Superfolder GFP was originally described by: Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006
 +
</ul>

Latest revision as of 20:49, 21 October 2019


lldRO1-J23117-lldRO2 Strong RBS sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 78
    Illegal NheI site found at 101
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 174


Design Notes

This part contains:

Source

sfGFP along with Terminator was obtained from the Biobrick. lldRO1-J23117-lldRO2 and B0034 was obtained as gBlock from IDT and these were joined together using SOEing (Spliced Overlap Extension) PCR.

References

  • parts.igem.org/Part:BBa_K1847008
  • Superfolder GFP was originally described by: Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006