Difference between revisions of "Part:BBa K3110040:Design"

 
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===Design Notes===
 
===Design Notes===
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None.
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This part contains:
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<ul>
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<li>The Promoter and Operator Region: lldRO1-J23117-lldRO2 ([[Part:BBa_K1847008]]) -designed by iGEM team of ETH Zurich -2015
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<li>A strong RBS: ([[Part:BBa_B0034]])
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<li>Reporter Protein: sfGFP ([[Part:BBa_I746916]])
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<li>Double terminator: ([[Part:BBa_I746916]])
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</ul>
  
 
===Source===
 
===Source===
  
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sfGFP was obtained from the Biobrick. lldRO1-J23100-lldRO2 were obtained as gBlocks from IDT and these were joined together using SOEing (Spliced Overlap Extenstion) PCR.
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sfGFP along with Terminator was obtained from the Biobrick. lldRO1-J23117-lldRO2 and B0034 was obtained as gBlock from IDT and these were joined together using SOEing (Spliced Overlap Extension) PCR.
  
 
===References===
 
===References===
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<ul>
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<li>parts.igem.org/Part:BBa_K1847008
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<li>Superfolder GFP was originally described by: Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006
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</ul>

Latest revision as of 20:49, 21 October 2019


lldRO1-J23117-lldRO2 Strong RBS sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 78
    Illegal NheI site found at 101
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 174


Design Notes

This part contains:

Source

sfGFP along with Terminator was obtained from the Biobrick. lldRO1-J23117-lldRO2 and B0034 was obtained as gBlock from IDT and these were joined together using SOEing (Spliced Overlap Extension) PCR.

References

  • parts.igem.org/Part:BBa_K1847008
  • Superfolder GFP was originally described by: Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006