Difference between revisions of "Part:BBa K3110040:Design"

(Design Notes)
(Design Notes)
Line 9: Line 9:
 
This part contains:
 
This part contains:
 
<ul>
 
<ul>
<li>The Promoter and Operator Region: lldRO1-J23117-lldRO2 ([[Part:BBa_K1847008]])
+
<li>The Promoter and Operator Region: lldRO1-J23117-lldRO2 ([[Part:BBa_K1847008]]) -designed by iGEM team of ETH Zurich -2015
 
<li>A strong RBS: ([[Part:BBa_B0034]])
 
<li>A strong RBS: ([[Part:BBa_B0034]])
 
<li>Reporter Protein: sfGFP ([[Part:BBa_I746916]])
 
<li>Reporter Protein: sfGFP ([[Part:BBa_I746916]])

Revision as of 20:40, 21 October 2019


lldRO1-J23117-lldRO2 Strong RBS sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 78
    Illegal NheI site found at 101
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 174


Design Notes

This part contains:

Source

sfGFP along with Terminator was obtained from the Biobrick. lldRO1-J23117-lldRO2 and B0034 was obtained as gBlock from IDT and these were joined together using SOEing (Spliced Overlap Extension) PCR.

References