Difference between revisions of "Part:BBa K3110040:Design"

(Design Notes)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
None.
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This part contains:
 
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<ul>
 
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<li>The Promoter and Operator Region: lldRO1-J23117-lldRO2 ([[Part:BBa_K1847008]])
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<li>A strong RBS: ([[Part:BBa_B0034]])
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<li>Reporter Protein: sfGFP ([[Part:BBa_I746916]])
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<li>Double terminator: ([[Part:BBa_I746916]])
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</ul>
  
 
===Source===
 
===Source===

Revision as of 17:27, 21 October 2019


lldRO1-J23117-lldRO2 Strong RBS sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 78
    Illegal NheI site found at 101
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 174


Design Notes

This part contains:

Source

sfGFP along with Terminator was obtained from the Biobrick. lldRO1-J23117-lldRO2 and B0034 was obtained as gBlock from IDT and these were joined together using SOEing (Spliced Overlap Extension) PCR.

References