Difference between revisions of "Help:Plasmid backbones"

Plasmids are circular, double-stranded DNA molecules typically containing a few thousand base pairs that replicate within the cell independently of the chromosomal DNA. Plasmid DNA is easily purified from cells, manipulated using common lab techniques and incorporated into cells. Most BioBrick parts in the Registry are maintained and propagated on plasmids. Thus, construction of BioBrick parts, devices and systems usually requires working with plasmids.

• Glossary - What are plasmids, vectors and backbones?

• Plasmid features - Common features of plasmids

• Plasmid naming - How BioBrick vectors are named

• Choosing the right plasmid - Choosing which plasmid to use

• Finding plasmids - How to locate plasmids in the Registry

• Constructing new plasmids - How to construct new BioBrick plasmids

• Entering new plasmids in the Registry - How to enter new plasmid backbones in the Registry

• Browse plasmid parts

All Registry plasmids are available through the use of BBa_P1010, BBa_I52001 and BBa_I52002. (Information on how to use these parts is available on the relevant part page).