Difference between revisions of "Part:BBa J45400:Experience"

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===Applications of BBa_J45400===
 
===Applications of BBa_J45400===
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The production of isoamyl acetate was verified by controlled tests in which E. coli
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Isoamyl alcohol (banana odor precursor) production.
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with J45220 or J45200 but no J45400 did not produce isoamyl acetate (that is, they did
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not give off banana scent), while E. coli containing both the J45220 or J45200 and
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J45400 did produce isoamyl acetate autonomously.  The main application of this device is that when
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coupled with <bbpart>J45220</bbpart> or <bbpart>J45200</bbpart>, the device can allow E. coli to synthesize isoamyl acetate endogenously.
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===User Reviews===
 
===User Reviews===
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<partinfo>BBa_J45400 AddReview 1</partinfo><br>
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<I>[[User:Rshetty|Reshma Shetty]]</I>
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<partinfo>BBa_J45400</partinfo>, when used in the banana odor biosynthetic system <partinfo>BBa_J45900</partinfo>, may not be functional since no isoamyl acetate is detected.
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==Characterization==
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===Gas chromatography analysis of banana odor biosynthetic system===
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The banana odor biosynthetic system (<partinfo>BBa_J45900</partinfo>) includes the isoamyl alcohol generator (<partinfo>BBa_J45400</partinfo>). 
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{{:IGEM:MIT/2006/Banana odor biosynthetic system gas chromatograms}}
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Latest revision as of 19:44, 8 March 2008

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_J45400

Isoamyl alcohol (banana odor precursor) production.

User Reviews

UNIQaa06208df240954e-partinfo-00000000-QINU

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Reshma Shetty

BBa_J45400, when used in the banana odor biosynthetic system BBa_J45900, may not be functional since no isoamyl acetate is detected.

UNIQaa06208df240954e-partinfo-00000004-QINU

Characterization

Gas chromatography analysis of banana odor biosynthetic system

The banana odor biosynthetic system (BBa_J45900) includes the isoamyl alcohol generator (BBa_J45400).

No isoamyl acetate production from cellular metabolites by banana biosynthetic system
We designed and constructed a system for banana odor production from intracellular metabolites (BBa_J45900). We analyzed cultures with the banana odor biosynthetic system for isoamyl acetate production but did not measure detectable levels of isoamyl acetate by gas chromatography (A). The banana odor generator (BBa_J45200) in the absence of exogenous isoamyl acetate does not produce measurable quantities of isoamyl acetate (B). For reference, we analyzed a sample with 100 ppm pure isoamyl acetate as well (E). Most E. coli strains produce indole. Octyl acetate was used as an internal standard for all samples containing isoamyl acetate.