Difference between revisions of "Part:BBa J45319:Experience"
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| + | <I>[[User:Rshetty|Reshma Shetty]]</I> | ||
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| + | <partinfo>BBa_J45319</partinfo>, when used in the wintergreen odor biosynthetic system <partinfo>BBa_J45700</partinfo>, appears to produce some salicylate from the cellular metabolite chorismate. | ||
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Revision as of 02:47, 8 March 2008
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_J45319
User Reviews
UNIQ76c3242d00792edb-partinfo-00000000-QINU
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BBa_J45319, when used in the wintergreen odor biosynthetic system BBa_J45700, appears to produce some salicylate from the cellular metabolite chorismate. |
UNIQ76c3242d00792edb-partinfo-00000004-QINU
Characterization
Gas chromatography analysis of wintergreen odor biosynthetic system
The wintergreen odor biosynthetic system (BBa_J45700) includes the PchA & PchB enzyme generator (BBa_J45319) under the control of a constitutive promoter (BBa_R0011).
Verification of methyl salicylate production from cellular metabolites by wintergreen biosynthetic system
We successfully designed, constructed and tested a system for wintergreen odor production from intracellular metabolites (BBa_J45700). We analyzed cultures for methyl salicylate production using gas chromatography. The wintergreen odor biosynthetic system (BBa_J45700) produced some methyl salicylate (A). The wintergreen odor generator (BBa_J45120) in the absence of either the salicylate generator (BBa_J45320) or exogenous salicylic acid did not produce methyl salicylate (B). The retention time of the methyl salicylate peak from BBa_J45700 is identical to that of the pure methyl salicylate standard (C). Most E. coli strains produce indole.
We successfully designed, constructed and tested a system for wintergreen odor production from intracellular metabolites (BBa_J45700). We analyzed cultures for methyl salicylate production using gas chromatography. The wintergreen odor biosynthetic system (BBa_J45700) produced some methyl salicylate (A). The wintergreen odor generator (BBa_J45120) in the absence of either the salicylate generator (BBa_J45320) or exogenous salicylic acid did not produce methyl salicylate (B). The retention time of the methyl salicylate peak from BBa_J45700 is identical to that of the pure methyl salicylate standard (C). Most E. coli strains produce indole.