Difference between revisions of "Part:BBa J45017:Design"

(→‎References)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J45017 short</partinfo>
 
<partinfo>BBa_J45017 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
 +
PchA (isochorismate synthase) catalyzes the conversion of chorismate to isochorismate.  PchB (isochorismate-pyruvate lyase) catalyzes the conversion of isochorismate to salicylate.
  
−
Salicylate is made from chorismate via isochorismate. The reactions are catalysed by PchA (isochorismate synthase) and PchB (isochorismate-pyruvate lyase).  Combined expression of the two proteins from one BioBrick part successfully generates salicylate in ''E. coli.''  For additional design features see [http://parts.mit.edu/wiki/index.php/MIT_2006 the MIT iGEM 2006 wiki].
+
Co-expression of the two proteins from one BioBrick part successfully generates salicylate in ''E. coli''. For additional design features see [http://parts.mit.edu/wiki/index.php/MIT_2006 the MIT iGEM 2006 wiki].
  
 
===Source===
 
===Source===
−
 
+
PchB and PchA are found in ''Pseudomonas aeruginosa''.  The pME3366 plasmid from which <partinfo>BBa_J45017</partinfo> was constructed was kindly sent to the MIT 2006 iGEM team by Dr. Cornelia Reimmann, Department of Fundamental Microbiology, Universite de Lausanne.  Information about Dr. Reimmann's work is available at [http://www.unil.ch/dmf/page16594.html her website].
−
PchB and PchA are found in ''Pseudomonas aeruginosa''.  The plasmid was kindly sent to the MIT iGEM team by Dr. Cornelia Reimmann from the Universite de Lausanne.  Information about Dr. Reimmann's work is available at [http://www.unil.ch/dmf/page16594.html her website].
+
  
 
===References===
 
===References===
Line 18: Line 17:
  
 
<biblio>
 
<biblio>
−
# Serino-MolGenGenet-1995 pmid=7500944
+
#Serino-MolGenGenet-1995 pmid=7500944
 +
#Serino-JBacteriol-1997 pmid=8982005
 +
#Gaille-JBC-2002 pmid=11937513
 
#Gaille-JBC-2003 pmid=12624097
 
#Gaille-JBC-2003 pmid=12624097
 
#Zaitseva-JBC-2006 pmid=16914555
 
#Zaitseva-JBC-2006 pmid=16914555
 
</biblio>
 
</biblio>

Revision as of 20:07, 3 February 2008

isochorismate pyruvate-lyase and isochorismate synthase (pchBA); converts chorismate to salicylate


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 307
    Illegal NgoMIV site found at 711
    Illegal NgoMIV site found at 836
    Illegal NgoMIV site found at 847
    Illegal NgoMIV site found at 1128
    Illegal NgoMIV site found at 1596
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PchA (isochorismate synthase) catalyzes the conversion of chorismate to isochorismate. PchB (isochorismate-pyruvate lyase) catalyzes the conversion of isochorismate to salicylate.

Co-expression of the two proteins from one BioBrick part successfully generates salicylate in E. coli. For additional design features see [http://parts.mit.edu/wiki/index.php/MIT_2006 the MIT iGEM 2006 wiki].

Source

PchB and PchA are found in Pseudomonas aeruginosa. The pME3366 plasmid from which BBa_J45017 was constructed was kindly sent to the MIT 2006 iGEM team by Dr. Cornelia Reimmann, Department of Fundamental Microbiology, Universite de Lausanne. Information about Dr. Reimmann's work is available at [http://www.unil.ch/dmf/page16594.html her website].

References

Original paper describing pchBA Serino-MolGenGenet-1995. Information about the enzyme encoded by PchA Gaille-JBC-2003.

<biblio>

  1. Serino-MolGenGenet-1995 pmid=7500944
  2. Serino-JBacteriol-1997 pmid=8982005
  3. Gaille-JBC-2002 pmid=11937513
  4. Gaille-JBC-2003 pmid=12624097
  5. Zaitseva-JBC-2006 pmid=16914555

</biblio>