Difference between revisions of "Part:BBa I739003"

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===Part Structure===
 
===Part Structure===
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<p>The Biobrick encodes luxR ([https://parts.igem.org/wiki/index.php/Part:BBa_C0062 BBa_C0062]) under control of the constitutive promoter [https://parts.igem.org/wiki/index.php/Part:BBa_J23100 BBa_J23100] followed by the ribosome binding site [https://parts.igem.org/wiki/index.php/Part:BBa_B0034 BBa_B0034]. The transcription of luxR is terminated by the double terminator [https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015].</p>
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<p>The Biobrick encodes LuxR ([https://parts.igem.org/wiki/index.php/Part:BBa_C0062 BBa_C0062]) under control of the constitutive promoter [https://parts.igem.org/wiki/index.php/Part:BBa_J23105 BBa_J23105] followed by the ribosome binding site [https://parts.igem.org/wiki/index.php/Part:BBa_B0034 BBa_B0034]. The transcription of luxR is terminated by the double terminator [https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015].</p>
  
 
===Mode of Action===
 
===Mode of Action===
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<p>LacI binds to the pLac regulator ([https://parts.igem.org/wiki/index.php/Part:BBa_R0010 BBa_R0010)] (or to the PLlac01 hybrid regulator [https://parts.igem.org/wiki/index.php/Part:BBa_R0011 BBa_R0011] respectively) and represses transcription. If the inducer [http://openwetware.org/wiki/IPTG Isopropyl-beta-D-thiogalactopyranoside (IPTG)] is added, lacI action is inhibited and the promoter gets derepressed.</p>
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<p>Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL) (e.g. [https://parts.igem.org/wiki/index.php/Acyl-HSLs AHL]). This complex binds to a palindromic site on the promoter [https://parts.igem.org/wiki/index.php/Part:BBa_R0062 BBa_R0062], increasing the rate of transcription. So far, this [https://parts.igem.org/wiki/index.php/Lux LuxI/R] system is the best characterized system of all [https://parts.igem.org/wiki/index.php/Featured_Parts:Cell-Cell-Signaling cell-cell signaling systems].</p>
  
 
===Purpose===
 
===Purpose===
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<p>This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project description, this part is also termed ''Part 2''. The constitutively synthesized lacI interacts with .... A similar (but not used) construct in this context is [https://parts.igem.org/wiki/index.php/Part:BBa_Q04121 BBa_Q04121].</p>
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<p>This Biobrick was designed for the [http://2007.igem.org/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. When complexed with HSL, the constitutively synthesized LuxR interacts with the double promoters [https://parts.igem.org/wiki/index.php/Part:BBa_I739104 BBa_I739104] and [https://parts.igem.org/wiki/index.php/Part:BBa_I739105 BBa_I739105] which are parts of composites [https://parts.igem.org/wiki/index.php/Part:BBa_I739006 BBa_I739006] and [https://parts.igem.org/wiki/index.php/Part:BBa_I739007 BBa_I739007] respectively.</p>
  
 
===Testing===
 
===Testing===

Latest revision as of 19:51, 26 October 2007

Constitutive expression cassette for LuxR (J23100.B0034.C0062.B0015)

Part Structure

The Biobrick encodes LuxR (BBa_C0062) under control of the constitutive promoter BBa_J23105 followed by the ribosome binding site BBa_B0034. The transcription of luxR is terminated by the double terminator BBa_B0015.

Mode of Action

Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL) (e.g. AHL). This complex binds to a palindromic site on the promoter BBa_R0062, increasing the rate of transcription. So far, this LuxI/R system is the best characterized system of all cell-cell signaling systems.

Purpose

This Biobrick was designed for the [http://2007.igem.org/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. When complexed with HSL, the constitutively synthesized LuxR interacts with the double promoters BBa_I739104 and BBa_I739105 which are parts of composites BBa_I739006 and BBa_I739007 respectively.

Testing

Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]