Difference between revisions of "Part:BBa I739003"

(Mode of Action)
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===Mode of Action===
 
===Mode of Action===
<p>Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL) (e.g. [https://parts.igem.org/wiki/index.php/Acyl-HSLs AHL)]. This complex binds to a palindromic site on the promoter [https://parts.igem.org/wiki/index.php/Part:BBa_R0062 BBa_R0062], increasing the rate of transcription. This [https://parts.igem.org/wiki/index.php/Lux LuxI/R] system is the best characterized system of all [https://parts.igem.org/wiki/index.php/Featured_Parts:Cell-Cell-Signaling cell-cell signaling systems.</p>
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<p>Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL) (e.g. [https://parts.igem.org/wiki/index.php/Acyl-HSLs AHL)]. This complex binds to a palindromic site on the promoter [https://parts.igem.org/wiki/index.php/Part:BBa_R0062 BBa_R0062], increasing the rate of transcription. So far, this [https://parts.igem.org/wiki/index.php/Lux LuxI/R] system is the best characterized system of all [https://parts.igem.org/wiki/index.php/Featured_Parts:Cell-Cell-Signaling cell-cell signaling systems].</p>
  
 
===Purpose===
 
===Purpose===

Revision as of 12:56, 11 October 2007

Constitutive expression cassette for LuxR (J23100.B0034.C0062.B0015)

Part Structure

The Biobrick encodes luxR (BBa_C0062) under control of the constitutive promoter BBa_J23100 followed by the ribosome binding site BBa_B0034. The transcription of luxR is terminated by the double terminator BBa_B0015.

Mode of Action

Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL) (e.g. AHL). This complex binds to a palindromic site on the promoter BBa_R0062, increasing the rate of transcription. So far, this LuxI/R system is the best characterized system of all cell-cell signaling systems.

Purpose

This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project description, this part is also termed Part 2. The constitutively synthesized lacI interacts with .... A similar (but not used) construct in this context is BBa_Q04121.

Testing

Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]