Difference between revisions of "Part:BBa I739003"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_I739003 short</partinfo>
 
<partinfo>BBa_I739003 short</partinfo>
  
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The Biobrick encodes luxR (BBa_C0062) under control of the constitutive promoter (BBa_J23100) followed by the ribosome binding site BBa_B0034. The transcription of luxR is terminated by the double terminator BBa_B0015
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===Part Structure===
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<p>The Biobrick encodes [https://parts.igem.org/wiki/index.php/Help:Tag LVA]-tagged luxR ([https://parts.igem.org/wiki/index.php/Part:BBa_C0062 BBa_C0062]) under control of the constitutive promoter [https://parts.igem.org/wiki/index.php/Part:BBa_J23100 BBa_J23100] followed by the ribosome binding site [https://parts.igem.org/wiki/index.php/Part:BBa_B0034 BBa_B0034]. The transcription of luxR is terminated by the double terminator [https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015].</p>
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===Mode of Action===
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<p>LacI binds to the pLac regulator ([https://parts.igem.org/wiki/index.php/Part:BBa_R0010 BBa_R0010)] (or to the PLlac01 hybrid regulator [https://parts.igem.org/wiki/index.php/Part:BBa_R0011 BBa_R0011] respectively) and represses transcription. If the inducer [http://openwetware.org/wiki/IPTG Isopropyl-beta-D-thiogalactopyranoside (IPTG)] is added, lacI action is inhibited and the promoter gets derepressed.</p>
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===Purpose===
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<p>This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project description, this part is also termed ''Part 2''. The constitutively synthesized lacI interacts with .... A similar (but not used) construct in this context is [https://parts.igem.org/wiki/index.php/Part:BBa_Q04121 BBa_Q04121].</p>
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===Testing===
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<p>Checked for uniqueness of restriction enzyme cleavage sites:<br>
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Eco: ok<br>
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Xba: ok<br>
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Spe: ok<br>
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Pst: ok<br>
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</p>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:29, 11 October 2007

Constitutive expression cassette for LuxR (J23100.B0034.C0062.B0015)

Part Structure

The Biobrick encodes LVA-tagged luxR (BBa_C0062) under control of the constitutive promoter BBa_J23100 followed by the ribosome binding site BBa_B0034. The transcription of luxR is terminated by the double terminator BBa_B0015.

Mode of Action

LacI binds to the pLac regulator (BBa_R0010) (or to the PLlac01 hybrid regulator BBa_R0011 respectively) and represses transcription. If the inducer [http://openwetware.org/wiki/IPTG Isopropyl-beta-D-thiogalactopyranoside (IPTG)] is added, lacI action is inhibited and the promoter gets derepressed.

Purpose

This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project description, this part is also termed Part 2. The constitutively synthesized lacI interacts with .... A similar (but not used) construct in this context is BBa_Q04121.

Testing

Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]