Difference between revisions of "Part:BBa I739001"
Stefan Luzi (Talk | contribs) |
Stefan Luzi (Talk | contribs) |
||
Line 2: | Line 2: | ||
<partinfo>BBa_I739001 short</partinfo> | <partinfo>BBa_I739001 short</partinfo> | ||
− | The Biobrick encodes LVA-tagged tetR ([https://parts.igem.org/wiki/index.php/Part:BBa_C0040 BBa_C0040]) under control of the constitutive promoter | + | --Part Structure-- |
+ | The Biobrick encodes LVA-tagged tetR ([https://parts.igem.org/wiki/index.php/Part:BBa_C0040 BBa_C0040]) under control of the constitutive promoter [https://parts.igem.org/wiki/index.php/Part:BBa_J23100 BBa_J23100], followed by the ribosome binding site [https://parts.igem.org/wiki/index.php/Part:BBa_B0034 BBa_B0034]. The transcription of tetR is terminated by the double terminator [https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015].<br><br> | ||
TetR binds to the pTet regulator ([https://parts.igem.org/wiki/index.php/Part:BBa_R0040 BBa_R0040)] and represses its action. If the inducer [http://openwetware.org/wiki/ATc anhydrotetracycline (ATc)] is added, tetR action is inhibited and the promoter gets derepressed. | TetR binds to the pTet regulator ([https://parts.igem.org/wiki/index.php/Part:BBa_R0040 BBa_R0040)] and represses its action. If the inducer [http://openwetware.org/wiki/ATc anhydrotetracycline (ATc)] is added, tetR action is inhibited and the promoter gets derepressed. | ||
Revision as of 20:17, 10 October 2007
Constitutive expression cassette for TetR +LVA (J23100.B0034.C0040.B0015)
--Part Structure--
The Biobrick encodes LVA-tagged tetR (BBa_C0040) under control of the constitutive promoter BBa_J23100, followed by the ribosome binding site BBa_B0034. The transcription of tetR is terminated by the double terminator BBa_B0015.
TetR binds to the pTet regulator (BBa_R0040) and represses its action. If the inducer [http://openwetware.org/wiki/ATc anhydrotetracycline (ATc)] is added, tetR action is inhibited and the promoter gets derepressed.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]