Difference between revisions of "Part:BBa C0052"

Revision as of 21:45, 11 April 2007

cI repressor from phage 434 (+LVA)

The 434 cI repressor protein coding sequence is a 710 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the 434 regulatory sequence, Part:BBa_R0052. The sequence contains a LVA tag for faster degradation and has no RBS.

Usage and Biology

434 cI is a member of the lamboid cI protein family.

The coding sequence has been modified from wild type cI to contain an LVA tag and two TAA stop codons; its EcoRI recognition site has been mutated out by changing base 36 from T to C to be BioBrick-compatible.

Sequence and Features

Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 688
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 21:44, 11 April 2007 (view source) Scmohr (Talk \| contribs) (→‎Usage and Biology) ← Older edit		Revision as of 21:45, 11 April 2007 (view source) Scmohr (Talk \| contribs) (→‎Usage and Biology) Newer edit →
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	434 cI is a member of the lamboid cI protein family.		434 cI is a member of the lamboid cI protein family.

−	The coding sequence has been modified from wild type cI to contain an LVA [[Help:Tag\|tag]], two TAA stop codons~~, and~~ its EcoRI recognition site has been mutated out by changing base 36 from T to C to be BioBrick-compatible.	+	The coding sequence has been modified from wild type cI to contain an LVA [[Help:Tag\|tag]] and two TAA stop codons; its EcoRI recognition site has been mutated out by changing base 36 from T to C to be BioBrick-compatible.