Difference between revisions of "Part:BBa C0052"
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434 cI is a member of the lamboid cI protein family. | 434 cI is a member of the lamboid cI protein family. | ||
| − | The coding sequence has been modified from wild type cI to contain an LVA [[Help:Tag|tag]], | + | The coding sequence has been modified from wild type cI to contain an LVA [[Help:Tag|tag]], two TAA stop codons, and its EcoRI recognition site has been mutated out by changing base 36 from T to C to be BioBrick-compatible. |
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| − | two TAA stop codons, and its EcoRI recognition site has been mutated out | + | |
| − | + | ||
| − | by changing base 36 from T to C to be BioBrick-compatible. | + | |
Revision as of 21:44, 11 April 2007
cI repressor from phage 434 (+LVA)
The 434 cI repressor protein coding sequence is a 710 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the 434 regulatory sequence, Part:BBa_R0052. The sequence contains a LVA tag for faster degradation and has no RBS.
Usage and Biology
434 cI is a member of the lamboid cI protein family.
The coding sequence has been modified from wild type cI to contain an LVA tag, two TAA stop codons, and its EcoRI recognition site has been mutated out by changing base 36 from T to C to be BioBrick-compatible.
Sequence and Features
Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 688
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]