Difference between revisions of "Part:BBa C0052"
Line 2: Line 2:
 
<partinfo>BBa_C0052 short</partinfo>
 
<partinfo>BBa_C0052 short</partinfo>
  
−
The 434 cI repressor protein coding sequence is a 710 base-pair sequence with      the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix      sections on its ends. It binds to the 434 regulatory sequence, [[Part:BBa_R0052]].      The sequence contains a LVA tag for faster degredation and has no [[Help:Ribosome Binding Site|RBS]].
+
The 434 cI repressor protein coding sequence is a 710 base-pair sequence with      the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix      sections on its ends. It binds to the 434 regulatory sequence, [[Part:BBa_R0052]].      The sequence contains a LVA tag for faster degradation and has no [[Help:Ribosome Binding Site|RBS]].
  
  

Revision as of 21:43, 11 April 2007

cI repressor from phage 434 (+LVA)

The 434 cI repressor protein coding sequence is a 710 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the 434 regulatory sequence, Part:BBa_R0052. The sequence contains a LVA tag for faster degradation and has no RBS.


Usage and Biology

434 cI is a member of the lamboid cI protein family.

The coding sequence has been modified from wild type cI to contain an LVA tag,

two TAA stop codons, and its EcoRI recognition site has been mutated out

by changing base 36 from T to C to be BioBrick-compatible.


Sequence and Features


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 688
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]