Difference between revisions of "Part:BBa K1587006:Design"

Latest revision as of 21:03, 18 September 2015

pompC and lacI box regulated cI expression

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

Source

We used the sequence BBa_R0082 for pompC, BBa_R0010 for lacI bindig site, BBa_B0030 for the RBS and BBa_K327018 for cI.

References

- Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson EA, Scouras A, Ellington AD, Marcotte EM & Voigt CA (2005) Synthetic biology: Engineering Escherichia coli to see light. Nature 438: 441–442
- Lee JM, Lee J, Kim T & Lee SK (2013) Switchable Gene Expression in Escherichia coli Using a Miniaturized Photobioreactor. PLoS ONE 8: e52382
- Gambetta GA & Lagarias JC (2001) Genetic engineering of phytochrome biosynthesis in bacteria. PNAS 98: 10566–10571

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 __NOTOC__
 <partinfo>BBa_K1587006 short</partinfo>
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 ===Design Notes===
-No information
 ===Source===
-aa
+We used the sequence [https://parts.igem.org/Part:BBa_R0082 BBa_R0082] for pompC, BBa_R0010 for lacI bindig site, [https://parts.igem.org/Part:BBa_B0030 BBa_B0030] for the RBS and [https://parts.igem.org/Part:BBa_K327018 BBa_K327018] for cI.
 ===References===
+- Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson EA, Scouras A, Ellington AD, Marcotte EM & Voigt CA (2005) Synthetic biology: Engineering Escherichia coli to see light. Nature 438: 441–442<br>
+- Lee JM, Lee J, Kim T & Lee SK (2013) Switchable Gene Expression in Escherichia coli Using a Miniaturized Photobioreactor. PLoS ONE 8: e52382<br>
+- Gambetta GA & Lagarias JC (2001) Genetic engineering of phytochrome biosynthesis in bacteria. PNAS 98: 10566–10571<br>