Difference between revisions of "Part:BBa K1475008:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1475008 short</partinfo>
 
<partinfo>BBa_K1475008 short</partinfo>
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===Design Notes===
 
===Design Notes===
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This part was constructed using PCR to remove the rapid degradation tag from the lacI+LVA registry part ([https://parts.igem.org/Part:BBa_C0012 Part:BBa_C0012]). In addition to removing the rapid degradation tag, promoter, RBS and terminator was added with the following primers:
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<br>
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Forward (containing: prefix, [https://parts.igem.org/Part:BBa_J23106 Pcon:BBa_J23106] and [https://parts.igem.org/Part:BBa_B0030 RBS:BBa_B0030]):
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<br>
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AAGAATTCGCGGCCGCTTCTAGAGTTTACGGCTAGCTCAGTCCTAGGTATAGTGCTAGCATTAAAGAGGAGAAATACTAGATGAAACCAGTAACGTTATACGATGT
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<br>
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Reverse (containing: suffix and [https://parts.igem.org/Part:BBa_B1002 terminator:BBa_B1002]):
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<br>
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TTTCTGCAGCGGCCGCTACTAGTAGCGAAAAAACCCCGCCGAAGCGGGGTTTTTTGCGTTATTACTGCCCGCTTTCCAGT
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===Source===
 
===Source===
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lacI repressor from ''Escherichia coli''
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===References===
 
===References===
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UniProt accesstion for ''Escherichia coli'' lacI: http://www.uniprot.org/uniprot/P03023
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<br>
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Entrez gene accession for ''Escherichia coli'' lacI: http://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=Graphics&list_uids=945007
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<br>
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Protein database entry for ''Escherichia coli'' lacI: http://www.rcsb.org/pdb/explore/explore.do?pdbId=1CJG

Latest revision as of 12:48, 16 October 2014

lacI protein produced by constitutive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was constructed using PCR to remove the rapid degradation tag from the lacI+LVA registry part (Part:BBa_C0012). In addition to removing the rapid degradation tag, promoter, RBS and terminator was added with the following primers:
Forward (containing: prefix, Pcon:BBa_J23106 and RBS:BBa_B0030):
AAGAATTCGCGGCCGCTTCTAGAGTTTACGGCTAGCTCAGTCCTAGGTATAGTGCTAGCATTAAAGAGGAGAAATACTAGATGAAACCAGTAACGTTATACGATGT
Reverse (containing: suffix and terminator:BBa_B1002):
TTTCTGCAGCGGCCGCTACTAGTAGCGAAAAAACCCCGCCGAAGCGGGGTTTTTTGCGTTATTACTGCCCGCTTTCCAGT



Source

lacI repressor from Escherichia coli

References

UniProt accesstion for Escherichia coli lacI: http://www.uniprot.org/uniprot/P03023
Entrez gene accession for Escherichia coli lacI: http://www.ncbi.nlm.nih.gov/gene?cmd=Retrieve&dopt=Graphics&list_uids=945007
Protein database entry for Escherichia coli lacI: http://www.rcsb.org/pdb/explore/explore.do?pdbId=1CJG