Difference between revisions of "Part:BBa K1045017:Design"
(→Source) |
(→Source) |
||
| Line 10: | Line 10: | ||
===Source=== | ===Source=== | ||
| − | This composite part was constructed using hybridization oligos for [[Part:BBa_K1045010|BBa_1045010]],[[Part:BBa_K1045011|BBa_1045011]] and [[Part:BBa_K1045012|BBa_1045012]]. The hybridization oligos were ordered from Sigma-Aldrich. Sequence information derived from the parts registry and Zhang ''et al.'', 2013. In addition, parts that were obtained either by amplification of plasmid DNA from the distribution kit 2013(inverse terminator [[Part:BBa_K1045009|BBa_1045009]]) or ''M. smegmatis'' chromosomal DNA (DarR ORF, [[Part:BBa_K1045001|BBa_1045001]]) were employed. The chromosomal DNA of ''Mycobacterium smegmatis'' was purchased from DSMZ ([http://www.dsmz.de/catalogues/details/culture/DSM-43756.html?tx_dsmzresources_pi5%5BreturnPid%5D=304 DSM No. 43756]). Part [[Part:BBa_E0240|BBa_E0240]] derived from the plasmid in the distribution kit 2013. | + | This composite part was constructed using hybridization oligos for [[Part:BBa_K1045010|BBa_1045010]], [[Part:BBa_K1045011|BBa_1045011]] and [[Part:BBa_K1045012|BBa_1045012]]. The hybridization oligos were ordered from Sigma-Aldrich. Sequence information derived from the parts registry and Zhang ''et al.'', 2013. In addition, parts that were obtained either by amplification of plasmid DNA from the distribution kit 2013 (inverse terminator [[Part:BBa_K1045009|BBa_1045009]]) or ''M. smegmatis'' chromosomal DNA (DarR ORF, [[Part:BBa_K1045001|BBa_1045001]]) were employed. The chromosomal DNA of ''Mycobacterium smegmatis'' was purchased from DSMZ ([http://www.dsmz.de/catalogues/details/culture/DSM-43756.html?tx_dsmzresources_pi5%5BreturnPid%5D=304 DSM No. 43756]). Part [[Part:BBa_E0240|BBa_E0240]] derived from the plasmid in the distribution kit 2013. |
===References=== | ===References=== | ||
Lei Zhang, Weihui Li, and Zheng-Guo He (2013) “DarR, a TetR-like Transcriptional Factor, Is a Cyclic Di-AMP-responsive Repressor in ''Mycobacterium smegmatis''”, THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 288, NO. 5, pp. 3085–3096 | Lei Zhang, Weihui Li, and Zheng-Guo He (2013) “DarR, a TetR-like Transcriptional Factor, Is a Cyclic Di-AMP-responsive Repressor in ''Mycobacterium smegmatis''”, THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 288, NO. 5, pp. 3085–3096 | ||
Revision as of 14:24, 19 October 2013
DarR reporter system
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 806
Illegal NheI site found at 829
Illegal NheI site found at 909
Illegal NheI site found at 932
Illegal NotI site found at 718 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 731
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 860
Illegal AgeI site found at 168 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1630
Design Notes
DarR fused to the terminator was cut out from BBa_K1045016 and ligated in a pre-fixing composition into BBa_K1045015.
Source
This composite part was constructed using hybridization oligos for BBa_1045010, BBa_1045011 and BBa_1045012. The hybridization oligos were ordered from Sigma-Aldrich. Sequence information derived from the parts registry and Zhang et al., 2013. In addition, parts that were obtained either by amplification of plasmid DNA from the distribution kit 2013 (inverse terminator BBa_1045009) or M. smegmatis chromosomal DNA (DarR ORF, BBa_1045001) were employed. The chromosomal DNA of Mycobacterium smegmatis was purchased from DSMZ ([http://www.dsmz.de/catalogues/details/culture/DSM-43756.html?tx_dsmzresources_pi5%5BreturnPid%5D=304 DSM No. 43756]). Part BBa_E0240 derived from the plasmid in the distribution kit 2013.
References
Lei Zhang, Weihui Li, and Zheng-Guo He (2013) “DarR, a TetR-like Transcriptional Factor, Is a Cyclic Di-AMP-responsive Repressor in Mycobacterium smegmatis”, THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 288, NO. 5, pp. 3085–3096