Difference between revisions of "Part:BBa E0040:Experience"
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+ | <partinfo>BBa_E0040 AddReview 3</partinfo> | ||
+ | <I>USTC_China iGEM13</I> | ||
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+ | In USTC_China iGEM13's project,we bulit the N-terminal TD1 modified GFP to make it is capable of transporting through skin barrier, then induced expression in BL21 and Bacillus Subtilis WB800N with IPTG and tested positive by SDS-PAGE and ultraviolet ultraviolet spectrometry. Students in Wen lab validated the efficient transdermal function of N-terminal TD1 modified GFP(not BBa_E0040 ). | ||
+ | [[Image:USTC_China_iGEM13_BBa_K1074006_1.png|thumb|left|600px|figure 1 SDS-PAGE of N-terminal TD1 modified GFP]] | ||
+ | [[Image:USTC_China_iGEM13_BBa_K1074006_2.JPG|thumb|left|600px|figure 2 Fluorescence detection of N-terminal TD1 modified GFP expression with and without IPTG induction]] | ||
+ | [[Image:USTC_China_iGEM13_BBa_K1074000_4.jpg|thumb|left|600px|figure 3 Our Transdermal test device]] | ||
+ | [[Image:USTC_China_iGEM13_BBa_K1074006_3.JPG|thumb|left|600px|figure 4 Results of transdermal test of N-terminal TD1 modified GFP and unmodified GFP]] | ||
+ | ===Reference=== | ||
+ | SUN Zheng, WEN Long-ping.Transdermal delivery of fusion green fluorescent protein mediated by covalently associated TD1 peptide. | ||
+ | OURNAL OF UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA.2009.Vol.39,No.4 | ||
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Revision as of 09:01, 27 September 2013
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_E0040
User Reviews
UNIQ7548cb8a40d25336-partinfo-00000000-QINU
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USTC_China iGEM13 |
In USTC_China iGEM13's project,we bulit the N-terminal TD1 modified GFP to make it is capable of transporting through skin barrier, then induced expression in BL21 and Bacillus Subtilis WB800N with IPTG and tested positive by SDS-PAGE and ultraviolet ultraviolet spectrometry. Students in Wen lab validated the efficient transdermal function of N-terminal TD1 modified GFP(not BBa_E0040 ). ReferenceSUN Zheng, WEN Long-ping.Transdermal delivery of fusion green fluorescent protein mediated by covalently associated TD1 peptide. OURNAL OF UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA.2009.Vol.39,No.4
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KAIST_iGEM_2012 |
Figure 1. E.coli strain MG1655 expressing BBa_E0040 under control of BBa_K907004 after overnight culture. 3mL culture with M9 media in 14 mL round bottom tube(Top left two) and centrifuged cells in eppendorf tube(Bottom left two). The expression of BBa_0040 is clearly observed with naked eye after overnight culture.
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Antiquity |
This review comes from the old result system and indicates that this part worked in some test. |
UNIQ7548cb8a40d25336-partinfo-00000009-QINU
E0040 contains 2 dpnI sites. This is useful when using a part containing E0040 as a template for PCR because you can cut up the template via a dpnI digest. For this to work the cell strain that the template was purified from must be dam methylase+.
iGEM11_WHU_China |
This part was used by WHU_China team as a reporter of long-term oscillator. Information from the website of this part shows that its length is about 720bp; however, the result of restriction enzymes cleavage experiment showed that it is actually about 1kb. And after several attempts, we failed to assemble this part with other biobricks. |