Difference between revisions of "PBAD SPL"
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List of sequences:
 
List of sequences:
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==DTU pBAD SPL==
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{|class="sortable" style="border:3px solid black; width: 70%" align="center"  cellspacing="0"
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! style="background:#AAAAAA; color:black"  |Identifier
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! style="background:#AAAAAA; color:black"  |Sequence<sup>a</sup>
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! colspan="2" style="background:#AAAAAA; color:black"  |Measured Strength<sup>b</sup>
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|-
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|align="center"|<Name>Mut. 1</Name>
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|<font face="Courier" size="+1">ttgacagctagctcagtcctaggtataatgctagc</font>
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|align="center"|n/a
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|-
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|align="center"|<partinfo>J23100</partinfo>
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|<font face="Courier" size="+1">ttgac<font color="red">g</font>gctagctcagtcctaggta<font color="red">c</font>a<font color="red">g</font>tgctagc</font>
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|align="center"|1
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|-
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|align="center"|<partinfo>J23101</partinfo>
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|<font face="Courier" size="+1">tt<font color="red">t</font>acagctagctcagtcctaggtattatgctagc</font>
 +
|align="center"|0.70
 +
|-
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|align="center"|<partinfo>J23102</partinfo>
 +
|<font face="Courier" size="+1">ttgacagctagctcagtcctaggta<font color="red">c</font>t<font color="red">g</font>tgctagc</font>
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|align="center"|0.86
 +
|-
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|align="center"|<partinfo>J23103</partinfo>
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|<font face="Courier" size="+1"><font color="red">c</font>tga<font color="red">t</font>agctagctcagtcctagg<font color="red">g</font>attatgctagc</font>
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|align="center"|0.01
 +
|-
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|align="center"|<partinfo>J23104</partinfo>
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|<font face="Courier" size="+1">ttgacagctagctcagtcctaggtatt<font color="red">g</font>tgctagc</font>
 +
|align="center"|0.72
 +
|-
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|align="center"|<partinfo>J23105</partinfo>
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|<font face="Courier" size="+1">tt<font color="red">t</font>ac<font color="red">g</font>gctagctcagtcctaggta<font color="red">c</font>tatgctagc</font>
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|align="center"|0.24
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|-
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|align="center"|<partinfo>J23106</partinfo>
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|<font face="Courier" size="+1">tt<font color="red">t</font>ac<font color="red">g</font>gctagctcagtcctaggtat<font color="red">ag</font>tgctagc</font>
 +
|align="center"|0.47
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|-
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|align="center"|<partinfo>J23107</partinfo>
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|<font face="Courier" size="+1">tt<font color="red">t</font>ac<font color="red">g</font>gctagctcag<font color="red">c</font>cctaggtattatgctagc</font>
 +
|align="center"|0.36
 +
|-
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|align="center"|<partinfo>J23108</partinfo>
 +
|<font face="Courier" size="+1"><font color="red">c</font>tgacagctagctcagtcctaggtat<font color="red">a</font>atgctagc</font>
 +
|align="center"|0.51
 +
|-
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|align="center"|<partinfo>J23109</partinfo>
 +
|<font face="Courier" size="+1">tt<font color="red">t</font>acagctagctcagtcctagg<font color="red">g</font>a<font color="red">ctg</font>tgctagc</font>
 +
|align="center"|0.04
 +
|-
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|align="center"|<partinfo>J23110</partinfo>
 +
|<font face="Courier" size="+1">tt<font color="red">t</font>ac<font color="red">g</font>gctagctcagtcctaggta<font color="red">ca</font>atgctagc</font>
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|align="center"|0.33
 +
|-
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|align="center"|<partinfo>J23111</partinfo>
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|<font face="Courier" size="+1">ttgac<font color="red">g</font>gctagctcagtcctaggtat<font color="red">ag</font>tgctagc</font>
 +
|align="center"|0.58
 +
|-
 +
|align="center"|<partinfo>J23112</partinfo>
 +
|<font face="Courier" size="+1"><font color="red">c</font>tga<font color="red">t</font>agctagctcagtcctagg<font color="red">g</font>attatgctagc</font>
 +
|align="center"|0.00
 +
|-
 +
|align="center"|<partinfo>J23113</partinfo>
 +
|<font face="Courier" size="+1"><font color="red">c</font>tga<font color="red">tg</font>gctagctcagtcctagg<font color="red">g</font>attatgctagc</font>
 +
|align="center"|0.01
 +
|-
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|align="center"|<partinfo>J23114</partinfo>
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|<font face="Courier" size="+1">tt<font color="red">t</font>a<font color="red">tg</font>gctagctcagtcctaggta<font color="red">ca</font>atgctagc</font>
 +
|align="center"|0.10
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|-
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|align="center"|<partinfo>J23115</partinfo>
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|<font face="Courier" size="+1">tt<font color="red">t</font>a<font color="red">t</font>agctagctcag<font color="red">c</font>cct<font color="red">t</font>ggta<font color="red">ca</font>atgctagc</font>
 +
|align="center"|0.15
 +
|-
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|align="center"|<partinfo>J23116</partinfo>
 +
|<font face="Courier" size="+1">ttgacagctagctcagtcctagg<font color="red">g</font>a<font color="red">c</font>tatgctagc</font>
 +
|align="center"|0.16
 +
|-
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|align="center"|<partinfo>J23117</partinfo>
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|<font face="Courier" size="+1">ttgacagctagctcagtcctagg<font color="red">g</font>att<font color="red">g</font>tgctagc</font>
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|align="center"|0.06
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|-
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|align="center"|<partinfo>J23118</partinfo>
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|<font face="Courier" size="+1">ttgac<font color="red">g</font>gctagctcagtcctaggtatt<font color="red">g</font>tgctagc</font>
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|align="center"|0.56
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|-
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|}
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 +
 +
 +
 +
 +
 +
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{| class="wikitable"
 
{| class="wikitable"
 
|-
 
|-

Revision as of 16:47, 26 September 2013

Here the DTU iGEM team will present their work on characterizing a SPL of pBAD promoters.

List of sequences:

DTU pBAD SPL

Identifier Sequencea Measured Strengthb
<Name>Mut. 1</Name> ttgacagctagctcagtcctaggtataatgctagc n/a
BBa_J23100 ttgacggctagctcagtcctaggtacagtgctagc 1
BBa_J23101 tttacagctagctcagtcctaggtattatgctagc 0.70
BBa_J23102 ttgacagctagctcagtcctaggtactgtgctagc 0.86
BBa_J23103 ctgatagctagctcagtcctagggattatgctagc 0.01
BBa_J23104 ttgacagctagctcagtcctaggtattgtgctagc 0.72
BBa_J23105 tttacggctagctcagtcctaggtactatgctagc 0.24
BBa_J23106 tttacggctagctcagtcctaggtatagtgctagc 0.47
BBa_J23107 tttacggctagctcagccctaggtattatgctagc 0.36
BBa_J23108 ctgacagctagctcagtcctaggtataatgctagc 0.51
BBa_J23109 tttacagctagctcagtcctagggactgtgctagc 0.04
BBa_J23110 tttacggctagctcagtcctaggtacaatgctagc 0.33
BBa_J23111 ttgacggctagctcagtcctaggtatagtgctagc 0.58
BBa_J23112 ctgatagctagctcagtcctagggattatgctagc 0.00
BBa_J23113 ctgatggctagctcagtcctagggattatgctagc 0.01
BBa_J23114 tttatggctagctcagtcctaggtacaatgctagc 0.10
BBa_J23115 tttatagctagctcagcccttggtacaatgctagc 0.15
BBa_J23116 ttgacagctagctcagtcctagggactatgctagc 0.16
BBa_J23117 ttgacagctagctcagtcctagggattgtgctagc 0.06
BBa_J23118 ttgacggctagctcagtcctaggtattgtgctagc 0.56





Mutant name Sequence
Mut. 1 CTGACGACGCCCCTCTCCGCCCCTTAAAATGTCCA
Mut. 2 CTGACGTAATCATACCGCCGAAAGTATTATCATTA
Mut. 3 CTGACGCGACAACATTGCGTCCTATAAAATGCCGA