Difference between revisions of "Part:BBa K1114002:Design"

 
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<partinfo>BBa_K1114002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1114002 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_J23102 with EB fusion sites. See the <a href="https://parts.igem.org/Part:BBa_J23102">BBa_J23102</a> page for full information on the part.  
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This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_J23102 with AB fusion sites. See the <a href="https://parts.igem.org/Part:BBa_J23102">BBa_J23102</a> page for full information on the part.  
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This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part.
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The fusion site letters refer to 4bp fusion sites: A        = GGAG;        B        = TACT; C        = AATG; D        = AGGT;        E        = GCTT; F        = CGCT; G        = TGCC; H        = ACTA.
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Summary of modifications from original part:
 
Summary of modifications from original part:
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Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
 
  
  
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Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.  See <a href="https://parts.igem.org/Part:BBa_K783055">BBa_K783055 </a>.
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===Source===
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iGEM Distribution Kit
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===References===
  
 
===Source===
 
===Source===

Latest revision as of 18:35, 7 September 2013


The MoClo format of BBa_J23102 with AB fusion sites.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Design Notes

This is the MoClo formatted part of BBa_J23102 with AB fusion sites. See the BBa_J23102 page for full information on the part. This is a Level 0 MoClo part with flanking sites A on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055 .


Source

iGEM Distribution Kit


References

Source

iGEM Distribution Kit

References