Difference between revisions of "Part:BBa K528015"

Latest revision as of 22:34, 21 September 2011

Expression Adapter s00_45k

This part encodes expression adaptor designed to have strength 64,29% relative to B0034 and uniform expression level regardless of attached protein.

Clonning:

The lab work was supervised by:

The part is in RFC25 standard. It consists of BBa_J23100 promoter followed by BBa_B0034 RBS, 5 nucleotide spacer and short peptide.

Spacer and peptide were designed using in silico evolution to provide uniform expression level regardless of following protein coding sequence.

Summary of design algorithm:

Using RBSCalculator program we have determined that this part expression strength is 64,29% relative to BBa_B0034

All our measurement results are summarized in the table below:

Part Name	Description	Expression level in thousands of absolute RBSDesigner units	Expression level as %B0034+YFP
BBa_K528015	Expression Adapters s00_45k	45	64,29%
BBa_K528016	Expression Adapters s01_24k	24	34,29%
BBa_K528017	expression adapters s02_20k	20	28,57%
BBa_K528018	Expression Adapters s06_16k	16	22,86%
BBa_K528019	Expression Adapters s05_12k	12	17,14%
BBa_K528020	expression adapters s03_65k	65	92,86%
BBa_K528021	BBa_K528018+YFP measurement device	16	22,86%
reference	BBa_J23102+B0034+YFP (100% reference construct)	70	100,00%

For more info please look at Team Warsaw 2011 [http://2011.igem.org/Team:Warsaw/ExpressionAdaptors wiki page].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 8
Illegal NheI site found at 31
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
-===Construct design===
+{{ExpressionAdaptor|strength=64,29%}}
-To make truly standardize strength of RBS parts we propose a brand new part in synthetic biology - we call expression adapters. They are short sequences consisting of RBS, 5 bp spacer and a short (10 amino acid long) beginning of a protein. 45k is strength in Voight's virtual units.
-For more info please look at Team Warsaw 2011 http://2011.igem.org/Team:Warsaw/ExpressionAdaptors/Results
-===Authors===
-Clonning:
-*Paweł Urbański
-*Elżbieta Jankowska
-*Krzysztof Szczepaniak
-*Dorota Kaja Sabat
-The lab work was supervised by:
-*Michał Lower
-*Ania Olchowik