RNA
Part:BBa_K5242008
Designed by: Jianting Gong Group: iGEM24_Tsinghua-M (2024-09-24)
ogRNA-chk1
This part can be fully complementarily paired with the RNA of Chk1, thereby inducing editing of ADAR, and is the core part used to assemble into a sensor for detecting Chk1. The addition of four of these MS2 sequences facilitates ADAR recognition and editing.At the beginning and end of the fragment, we added E2A and LV2A peptides, respectively, allowing both fluorescent proteins to be separated from the intervening peptide throughout sensorRNA translation.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 122
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 102
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 122
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 122
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |