RNA

Part:BBa_K5242008

Designed by: Jianting Gong   Group: iGEM24_Tsinghua-M   (2024-09-24)
Revision as of 21:33, 1 October 2024 by Gongjt23 (Talk | contribs)


ogRNA-chk1

This part can be fully complementarily paired with the RNA of Chk1, thereby inducing editing of ADAR, and is the core part used to assemble into a sensor for detecting Chk1. The addition of four of these MS2 sequences facilitates ADAR recognition and editing.At the beginning and end of the fragment, we added E2A and LV2A peptides, respectively, allowing both fluorescent proteins to be separated from the intervening peptide throughout sensorRNA translation.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 122
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 102
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 122
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 122
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None