RNA

Part:BBa_K5242008

Designed by: Jianting Gong   Group: iGEM24_Tsinghua-M   (2024-09-24)


ogRNA-chk1

This part can be complementarily paired with Chk1 and contains an A-C mismatch site in the middle for the introduction of ADAR editing. And this part is the core part of the sensor RNA used to detect Chk1. The addition of four of these MS2 sequences facilitates ADAR recognition and editing. At the beginning and end of the fragment, we added E2A and LV2A peptides, respectively, allowing both fluorescent proteins to be separated from the intervening peptide throughout sensor RNA translation.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 122
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 102
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 122
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 122
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None