DNA
Part:BBa_K5061006
Designed by: Florent Cha Group: iGEM24_Evry-Paris-Saclay (2024-09-14)
Revision as of 22:11, 20 September 2024 by Chaflorent (Talk | contribs)
Golden Gate adapter for Accessory Plasmids (AP) Golden Gate cloning platforms for M13 gVI expression
This Golden Gate adapter contains two BsaI recognition sites in opposite direction.
It is present in our composite parts BBa_K5061032 and BBa_K5061132and allows rapid engineering using Golden Gate Assembly of Accessory Plasmids (AP) for phage-assisted continuous and non-continuous evolution (PACE and PANCE).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 26
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 18
Illegal BsaI.rc site found at 6
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Categories
Parameters
None |