Composite

Part:BBa_K5061132

Designed by: Florent Cha   Group: iGEM24_Evry-Paris-Saclay   (2024-09-14)


Accessory Plasmid (AP) Golden Gate cloning platform for M13 gIII and gVI expression

to do

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 5961
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 5961
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 697
    Illegal XhoI site found at 26
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 5961
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 5961
    Illegal AgeI site found at 2172
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 18
    Illegal BsaI.rc site found at 6
    Illegal SapI site found at 2691


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Categories
Parameters
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