DNA

Part:BBa_K5061005

Designed by: Florent Cha   Group: iGEM24_Evry-Paris-Saclay   (2024-09-14)
Revision as of 22:10, 20 September 2024 by Chaflorent (Talk | contribs)


Golden Gate adapter for Helper phages (HP) M13KO7-ΔgVI and M13KO7-ΔgIII-ΔgVI Golden G

This Golden Gate adapter contains two BsaI recognition sites in opposite direction.

It is present in our composite parts BBa_K5061031 and BBa_K5061131and allows rapid engineering using Golden Gate Assembly of helper phages (HP) for phage-assisted continuous and non-continuous evolution (PACE and PANCE).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 12
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 18
    Illegal BsaI.rc site found at 6


[edit]
Categories
Parameters
None