DNA
Part:BBa_K5061005
Designed by: Florent Cha Group: iGEM24_Evry-Paris-Saclay (2024-09-14)
Golden Gate adapter for Helper phages (HP) M13KO7-ΔgVI and M13KO7-ΔgIII-ΔgVI Golden G
This Golden Gate adapter contains two BsaI recognition sites in opposite direction.
It is present in our composite parts BBa_K5061031 and BBa_K5061131 and allows rapid engineering using Golden Gate Assembly of helper phages (HP) for phage-assisted continuous and non-continuous evolution (PACE and PANCE).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 12
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 18
Illegal BsaI.rc site found at 6
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Categories
Parameters
None |