Part:pSB1C00

Universal Acceptor for MoClo / Loop

Universal Acceptor for MoClo / Loop based on pSB1C3.

This plasmid backbone has two uses:

• Creating new Level 0 basic parts: When used with BBa_J04452, new Level 0 basic parts can be cloned into it, and selected against BBa_J04452 , an RFP device.
• Level 1 Assembly: Level 0 basic parts maintained in this plasmid can be assembled into a Level 1 (Odd) plasmid, using the flanking Bsa1 sites.

Usage and Biology

Universal Acceptor

When BBa_J04452 and pSB1C00 are paired together, pSB1C00 can be used as a Universal Acceptor to clone in new Level 0 basic parts made through PCR or synthesis. View the BBa_J04452's Get This Part page to find a sample in the distribution kit.

1. Add the following ends to your part sample through PCR or synthesis. This will result in your part flanked by the desired Type IIS fusion sites (numbered) and Sap1 sites.

2. When both your part sample and the whole acceptor plasmid (pSB1C00-BBa_J04452) are cut with Sap1, the RFP device will be excised, leaving a partial Bsa1 sequence. Your cut part sample will have the correct sticky ends to ligate in.

3. Ligated product will result in your part in the backbone and flanked by Bsa1 sites. You can select for your liagted part by screening against the RFP device.

More details to follow.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2041
  Illegal PstI site found at 8
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2041
  Illegal PstI site found at 8
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2041
  Illegal XhoI site found at 1025
  Illegal XhoI site found at 1917
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2041
  Illegal PstI site found at 8
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2041
  Illegal PstI site found at 8
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 2047
  Illegal BsaI.rc site found at 2