Reporter

Part:BBa_K895007

Designed by: Frank Sargent   Group: iGEM12_Dundee   (2012-09-23)

Tetrathionate-sensing two-component system linked to GFP production.

This is a biosensor comprising the engineered Salmonella TtrRS two-component system from BBa_K895003 linked to GFP from BBa_K895006, which was originally supplied in BBa_J54103. The concept is that, primarily under anaerobic conditions, the two-component system will sense tetrathionate and induce GFP production.

Results in Experience Page

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2550
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1179
    Illegal AgeI site found at 311
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 28
    Illegal BsaI.rc site found at 3199


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