Signalling

Part:BBa_K895003

Designed by: Frank Sargent   Group: iGEM12_Dundee   (2012-09-22)


Salty_TtrRS_two_component_system

This is a signalling system comprising two open reading frames of the ttrR and ttrS genes together with the promoter/operator of the ttrB gene from Salmonella LT2. Together, TtrR and TtrS form a canonical two-component system where TtrS is the membrane-bound sensor kinase and TtrR is the DNA-binding response regulator. The system senses tetrathionate outside the cell and activates transcription of the ttrB gene, which is part of a tetrathionate reducatase. This part has been designed so that expression of alternative reporter genes can be regulated by tetrathionate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2550
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1179
    Illegal AgeI site found at 311
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 28


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Categories
Parameters
None