LacI generator (no promoter)
A composite part with coding sequence for LacI-LVA together with ribosome binding site (RBS) and a double transcriptional terminator. For use in regulation of LacI-repressed genetic elements, by combining the part with an appropriate promoter.
This part is a duplicate of BBa_K292006. The NTNU iGEM 2011 team found that the physical DNA available from the registry for that part did not match the registered sequence , and we have therefore remade it. Sequencing confirms that this part has the desired sequence.
The sequencing results can be found here. The sequencing result shows that in the old biobrick, only the terminator is present, and no LacI or RBS. In our improved biobrick, both RBS, LacI and terminator are present.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 1168
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC