Primer

Part:BBa_K4624604

Designed by: Christina Malamou   Group: iGEM23_Thessaly   (2023-09-28)


FadR B3+B4 Reverse primer for GoldenBraid 2.0 cloning.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage

This part was designed for the addition of 3' appropriate overhangs to FadR (BBa_K4624005) compatible with the 3' of the B3+B4 position of the GoldenBraid 2.0 cloning grammar. This part is meant to be used with FadR B3+B4+(B5) Forward primer (BBa_K4624603).

Figure 1: Gel electrophoresis of the PCR products for the (1) FadR B3+B4 part with primers BBa_K4624604 and BBa_K4624603. Expected bands (bp): Lane 1: 754, L=1kb plus DNA ladder
The successful addition of the appropriate 3' and 5' overhangs was confirmed upon the succesful insertion of the part into the universal part domesticator plasmid pUPD2, which is compatible only with domesticated parts, such as the PCR product.

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Categories
Parameters
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