Part:BBa_K4624005

FadR - fatty acid metabolism regulator in Escherichia coli

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Biology

Escherichia coli FadR is a dimeric helix-turn-helix transcription factor that plays a dual role in fatty acid metabolism ^[1]. This protein represses the fad genes of fatty acid degradation (β-oxidation) and activates the fab genes of fatty acid synthesis (FAS). The DNA-binding activity of FadR is specifically antagonized by acyl-CoAs ^[2], and thus FadR acts as a sensor of fatty acid availability in the environment ^[3].

References

1. Henry MF, Cronan JE Jr. Escherichia coli transcription factor that both activates fatty acid synthesis and represses fatty acid degradation. J Mol Biol. 1991 Dec 20;222(4):843-9. doi: 10.1016/0022-2836(91)90574-p. PMID: 1722255.

2. Cronan JE Jr. In vivo evidence that acyl coenzyme A regulates DNA binding by the Escherichia coli FadR global transcription factor. J Bacteriol. 1997 Mar;179(5):1819-23. doi: 10.1128/jb.179.5.1819-1823.1997. PMID: 9045847; PMCID: PMC178900.

3. Zhang F, Carothers JM, Keasling JD. Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids. Nat Biotechnol. 2012 Mar 25;30(4):354-9. doi: 10.1038/nbt.2149. PMID: 22446695.