Part:BBa_K4438911

crp_trigger_6_w/oPol

crp_trigger_6_w/oPol (BBa_K4438911) is a single-stranded DNA having 25 nucleotides. The 5’ end of the sequence has a few bases that are complementary to part Aptamer_crp (BBa_K4438900) and the 3’ end has an ssDNA sense T7 promoter sequence. Figure 1A) shows the secondary structure and minimum free energy.

Usage and Biology

This part can bind to Aptamer_crp (BBa_K4438900) due to complementarity. Figure 1D) Shows the secondary structure of both parts hybridised at 37° Celsius. CRP binds with Aptamer_crp (BBa_K4438900) and displaces the crp_trigger_6_w/oPol (BBa_K4438911) [1]. This part has complete complementarity with part crp_Target_6(No part name specified with partinfo tag.). T7 polymerase binds and in-vitro transcription of the duplex forms multiple broccoli light-up aptamers. All three parts can be used to detect different concentrations of CRP.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

Liu, Z., Luo, D., Ren, F., Ran, F., Chen, W., Zhang, B., ... & Chen, Q. (2019). Ultrasensitive fluorescent aptasensor for CRP detection based on the RNase H assisted DNA recycling signal amplification strategy. RSC advances, 9(21), 11960-11967.