Part:BBa_K4370005

RBS_PRK_STRBO

This parts encodes the phosphoribulokinase (PRK) of Streptomyces bottropensis ATCC 25435 (BBa_K4370004) under the control of an RBS (BBa_B0034). The co-expression of this part with the form I RuBisCO large (BBa_K4370000, BBa_K4370002) and small (BBa_K4370001, BBa_K4370003) subunits of the RuBisCO of this strain may help to implement the Calvin cycle in bacterial chassis of interest such as E. coli .

Usage and Biology

The PRK (phosphoribulokinase or ribulose-5-phosphate kinase) is a phosphotransferase that catalyze the synthesis of RuBP (D-ribulose-1,5-bisphosphate) using ATP and D-ribulose-5-phosphate as a substrate.

This enzyme closes the Calvin cycle by regenerating RuBP, the substrate of the RuBisCO. Its activity plays a key role in directing glucose to the RuBisCO-based pathway. PRK expression without RuBisCO may be toxic to cells. This observation is the basis for phenotypic assays to characterize the activity of PRK and/or RuBisCO (when both are introduced into cells) (Parikh et al., Protein Eng Des Sel, 2007).

Figure : Reaction catalyzed by the PRK

References Monal R. Parikh, Dina N. Greene, Kristen K. Woods, and Ichiro Matsumura, “Directed evolution of RuBisCO hypermorphs through genetic selection in engineered E.coli”, Protein Engineering, Design and Selection 2006 Mar; 19(3): 113–119 https://doi-org.insb.bib.cnrs.fr/10.1093/protein/gzj010

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]