Measurement

Part:BBa_K4133022

Designed by: Luis Alejandro Chaves Martinez   Group: iGEM22_Costa_Rica   (2022-10-04)


P48 + Lactobacillus rhamnosus GG RBS + GFP + Double Terminator

This part was created by combining the following parts:A P48 constitutive promoter (BBa_K1130001), a Lactobacillus rhamnosus GG RBS (BBa_K2760006), a GFP codon optimized for L. casei (BBa_K4133005) and the double terminator T7 and T1rrb (BBa_K3183021). The combination of these parts was performed with the aim to characterize the influence of different regulatory sequences and their combination on recombinant gene expression in Lactobacillus casei ATCC 393.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 678
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None