Tag

Part:BBa_K3757008

Designed by: Erik Zimmer   Group: iGEM21_Tuebingen   (2021-09-26)


c-myc tag, C-terminal

c-myc tag, containing a stop codon, for C-terminal tagging of proteins.


Usage and Biology

team Tuebingen 2021

The c-myc-tag 10 aa long affinity tag, which is widely used on recombinant proteins 1 . We tagged our Oak1 precursor (BBa_K3757001) C-terminally with the c-myc-tag in the composite part 35S Oak1 (BBa_K3757007). Even though we didn't use the tag for affinity purification or immunodetection, it has great potential to get characterised by future iGEM teams as a potent affinity tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None