Regulatory
Part:BBa_K3505015
Designed by: Venetios Michelioudakis, Magdalini Koroxenidou, Foteini Papadaki Group: iGEM20_Thessaly (2020-10-20)
prpBCDE:RBS GB compatible with A1-B2
Level 0 prpBCDE with RBS BBa_B0034
Usage and Biology
The prpBCDE is a SCFAs inducible promoter and more specifically from Acetate and Propionate[1]. From the prpBCDE operon at Salmonella typhimurium LT2.
Design Notes
The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 BBa_K3505007and has overhangs compatible for GoldenBraid cloning. The Promoter has position A1-B3.
Verification of cloning
Experimental Use and Experience
This part is used in BBa_K3505031
Source
Synthesized by IDT
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- [1]Tsang, A., Horswill, A. and Escalante-Semerena, J., 1998. Studies of Regulation of Expression of the Propionate (prpBCDE) Operon Provide Insights into How Salmonella typhimurium LT2 Integrates Its 1,2-Propanediol and Propionate Catabolic Pathways. Journal of Bacteriology, 180(24), pp.6511-6518.
[edit]
Categories
Parameters
None |