DNA
PmeI site

Part:BBa_K316013

Designed by: IC 2010 Team   Group: iGEM10_Imperial_College_London   (2010-10-23)

8bp recognition sequence for PmeI restriction endonuclease

Information about PmeI restriction endonuclease is available at [http://www.neb.com/nebecomm/products/productR0560.asp Neb website]1. The recognition site is a 8bp sequence GTTTAAAC. Pme produces a blunt cut after GTTT.

This is a planning part. The sequence was combined with BBa_K316000 to produce BBa_K316014 in construction of B. subtilis genome integration vectors


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

<biblio>

  1. 1 http://www.neb.com/nebecomm/products/productR0560.asp
  1. 2 http://2010.igem.org/Team:Imperial_College_London

</biblio>

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Categories
Parameters
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