Composite

Part:BBa_K2285015

Designed by: Zhuoyang Chen   Group: iGEM17_SJTU-BioX-Shanghai   (2017-10-18)


J23119+Target3+amilCP(RBS+amilCP+Term)

Constitutive promoter J23119 followed by a stem-loop structure Target3, with chromoprotein amilCP as the reporter gene. The original part of amilCP chromoprotein is presented on BBa_K1357009, which is a composite part with B0034 and B0010+B0012 double-terminator.

Usage and Biology

The coding region of amilGFP was originated from iGEM14_Virginia.

More infomation and details about usage of this part can refer to J23119+Target3+eforRed BBa_K2285012

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None